Evolutionary Epigenomics of Drosophila

 

Epigenetic gene regulation is one of the most exciting topics in biology today. The Y chromosome of Drosophila offers a unique opportunity to connect epigenetic regulation to specific adaptive traits. This 40 Mb chromosome is gene-poor and degenerate, with very little polymorphism in unique sequences owing to lack of recombination, exclusive transmission through males, and reduced effective population size. The Y chromosome has long been thought to have little role in phenotypic variation beyond a few Y-linked male fertility factors. We have described a new phenomenon termed Y-linked regulatory variation that affects the level of expression of hundreds of genes across the genome. We have shown that (1) these effects are largely, if not exclusively, epigenetic effects of microsatellites and other repeated sequences on the Y; and (2) Y chromosomes from natural populations are polymorphic for their effects on autosomal and X chromosomal gene expression. We have also shown that Y chromosomes from closely related species show Y-linked regulatory divergence and that these effects are associated with differences in male reproductive fitness. This project provides the first opportunities to associate specific repetitive DNA sequences on the Y chromosome to specific adaptive traits, to identify the networks of genes that are responsive to Y-linked regulatory variation, and to study Y-linked regulatory divergence and its association with male fitness in regard to implications for speciation.

 

  • Sackton, T. B., H. Montenegro, D. L. Hartl, and B. Lemos, 2011 Disruption of testis-specific gene expression and male reproductive phenotypes in heterospecific Y chromosome introgressions in Drosophila. Proc. Natl. Acad. Sci. USA 108: 17046-17051.
  • Paredes, S., A. T. Branco, D. L. Hartl, K. A. Maggert and B. Lemos 2011 Ribosomal DNA deletions modulate genome-wide gene expression: "rDNA-sensitive" genes and natural variation. PLoS Genetics 7: e1001376.
  • Jiang, P.-P., D. L. Hartl, and B. Lemos, 2010 Y not a dead end: Epistatic interactions between Y-linked regulatory polymorphisms and genetic background affect global gene expression. Genetics 186: 109-118. PMID: 20551438.
  • Lemos, B., A. Branco and D. L. Hartl, 2010 Epigenetic effects of polymorphic Y chromosomes modulate chromatin components, immune response, and sexual conflict. Proc. Natl. Acad. Sci. USA. 107: 15826-15831. PMID: 20798037.
  • Lemos, B., L. O. Araripe, and D. L. Hartl. 2008. Polymorphic Y chromosomes harbor cryptic variation with manifold functional consequences. Science 319: 91-93.