Active Research Evolutionary genomics of Drosophila Genetic diversity of P. falciparum Evolution of hybrid incompatibilities Evolution of gene expression Natural selection and protein evolution   Past Research Evolution of novel gene functions Bioinformatics Selective sweep C-value paradox Transposable element mariner Structure and sequence of the Drosophila genome Genome structure and evolution in D. virilis Cosuppression of transposable elements Molecular evolution in enteric bacteria Gregor Mendel

Structure and sequence of the Drosophila genome Our laboratory initiated the first large-scale physical mapping of the Drosophila genome which ultimately culminated in the complete sequence of the genome of D. melanogaster by Celera, Inc. The initial projects made use of large-fragment clones, particular bacteriophage P1 (PAC) and yeast artificial chromosome (YAC) clones. The most useful for our purposes proved to be PAC clones containing DNA fragments from D. melanogaster in the size range 75-100 kilobase pairs. The genomic location from which each of these clones derived was determined by in situ hybridization with polytene salivary-gland chromsomes. At the completion of the project we had mapped 2467 PAC and 1193 YAC clones and had achieved virtually complete coverage of the euchromatic portion of the genome.   PDF Hartl, D. L. 2000 Fly meets shotgun: Shotgun wins. Nature Genetics 24: 327-328.       Hartl, D. L. and E. R. Lozovskaya, 1995 The Drosophila Genome Map: Practical Guide. R. G. Landes Company, Austin Texas. 240 pp.       Segarra, C., E. R. Lozovskaya, G. Ribo, M. Aguade, and D. L. Hartl, 1995 P1 clones from Drosophila melanogaster as markers to study chromosomal evolution of Muller's A element in two species of the obscura group of Drosophila. Chromosoma 104: 129-136.     PDF Hartl, D. L., D. I. Nurminsky, R. W. Jones, and E. R. Lozovskaya, 1994 Genome structure and evolution in Drosophila: Applications of the framework P1 map. Proc. Natl. Acad. Sci. USA 91: 6824-6829.       Hartl, D. L. and E. R. Lozovskaya, 1992 The Drosophila genome project: Current status of the physical map. Comp. Biochem. Physiol 103B: 1-8.     PDF Merriam, J., M. Ashburner, D. L. Hartl and F. C. Kafatos, 1991 Toward cloning and mapping the genome of Drosophila. Science 254: 221-225.     PDF Garza, D., J. W. Ajioka, D. T. Burke and D. L. Hartl, 1989 Mapping the Drosophila genome with yeast artificial chromosomes. Science 246: 641-646.