Evolution of hybrid incompatibilities in the formation of animal species
Dobzhansky and Muller suggested that the normal course of evolution within isolated or semi-isolated populations gives rise to genetic incompatibilities that cause hybrid sterility or inviability and hence new species. Because most species are reproductively isolated by multiple genetic incompatibilities, few experimental systems have been developed in which the genes underlying the incompatibilities can be isolated individually. One promising system makes use of introgression, in which various sized pieces of the genome of one species are introduced into the genome of another species. In our case the species are Drosophila mauritiana and D. simulans. The biological resource represented by these introgression lines provides the material to isolate and identify specific genes that are associated with hybrid male sterility in order to determine their origin (point mutation, gene fusion, transposition, duplication), their molecular evolution, and their misexpression in hybrids.
Recently we discovered a cryptic system of sex-ratio distortion in D. simulans. In this case, a newly evolved X-linked gene we call Dox evolved the ability to distort the X chromosome in XY males, with the result that a Dox-bearing male produces about 80 percent female progeny. At the population level, this level of distortion imposes strong selection for suppressors. Consistent with this prediction, we have also discovered a newly evolved third-chromosomal gene we call Nmy that suppresses Dox. The Nmy suppressor appears to encode a microRNA. We are currently studying the mechanism and evolution of Dox and Nmy, and have evidence for a very recent strong selective sweep in the region of both genes.